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Results 1 - 6 of 6
EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.8analysis of the X-ray structure at 2.0 A resolution 657677
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.8crystal structure exhibits a classic thioredoxin-glutaredoxin fold comprising three alpha-helices surrounding four antiparallel beta-sheets. The crystal lattice has four monomers in a dimer of dimers arrangement. A cadmium ion is found within the active site of each monomer, and two cadmium ions stabilize the N-terminal tails and dimer interfaces. CoMSH and glutathione bind to the active site of methanoredoxin similar to the binding of glutathione in glutaredoxin 741927
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.8hanging-drop vapour diffusion method 677156
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.8purified recombinant His-tagged enzyme LpdA, hanging drop vapour diffusion method at room temperature, 0.003 ml protein solution containing 25 mg/ml protein, 10 mM HEPES, pH 7.5, mixed with 0.002 ml precipitant solution containing 0.1 M trisodium citrate, pH 5.4-5.8, 2.5-5.0% PEG 6000, 3-7 days depending on the pH, crystallization takes longer at higher pH-value, crystals are immersed in 10% PEG 6000, 100 mM MES, pH 5.75, and 25% glycerol, X-ray diffraction structure determination and analysis at 2.8 A resolution 659424
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.8sitting drop vapor diffusion method, using 10% (w/v) PEG 8000, 100 mM HEPES (pH 7.5), and 8 mM cystin at 20°C 725973
Display the word mapDisplay the reaction diagram Show all sequences 1.8.1.8sitting-drop vapor diffusion method, crystals belong to the hexagonal space group P6522 with cell dimensions of a = b = 110.3 and c = 68.5 and with one molecule in the asymmtric unit. The structure is solved by multiple isomorphous replacement including anomalous scattering data. The structure is refined to 1.9 resolution 723101
Results 1 - 6 of 6