EC Number   |
|---|
   1.7.6.1 | crystal structure of nitrophorin 4 at 1.5 A resolution |
| 1.7.6.1 | mutant enzyme E27V, vapor diffusion method, using 0.33% (w/v) anthrone, 0.33% (w/v) Congo Red, 0.33% (w/v) N-(2-acetamido)-2-aminoethanesulfonic acid, 0.0 2M HEPES sodium pH 6.8 |
| 1.7.6.1 | protein crystals are obtained using the vapor diffusion method with the conditions containing 3.2 M ammonium phosphate (pH 7.4). X-ray crystallography of NP4 crystals soaked with nitrite reveals the formation of an eta1-N nitro complex |
| 1.7.6.1 | solid-state NMR analysis. Enzyme tends to form oligomers and precipitates at higher concentration. At concentrations of approximately 0.1-2 mM oligomers are in equilibrium with monomers. The protein oligomerizes preferably in units of trimers, hexamers, and higher oligomeric states of unidentified composition |
