EC Number   |
|---|
   1.3.1.91 | mutant enzyme E294K, vapor diffusion method, using 30% (w/v) PEG 2000 MME, 200 mM ammonium sulfate, and 50 mM sodium acetate pH 5.5. Mutant enzyme E294K/Q305K, vapor diffusion method, using 30% (w/v) PEG 2000 MME, 200 mM ammonium sulfate, and 50 mM sodium acetate pH 5.0. Mutant enzyme Q305K, vapor diffusion method, using 2.2 M ammonium sulfate |
| 1.3.1.91 | purified recombinant catalytic HsDus2dusD domain and tRNA binding domain HsDus2dsRBD (Glu347-Lys451), by hanging drop vapor diffusion method, at 19°C, X-ray diffraction structure determination and crystal structure analysis, PDB IDs 4WFS and 4WFT |
| 1.3.1.91 | purified recombinant hDus2 catalytic and tRNA-recognition domains (residues 1-340), sitting drop vapour diffusion method, 300 nl of 10 mg/ml protein in 20 mM Tris, pH 8.0, 100 mM NaCl, 5 mM imidazole, and 5 mM DTT, are mixed with 0. 54 ml of reservoir solution containing 0.1 M MES-malic acid-Tris, pH 4.0, and 25% w/v PEG 1500, 2 days, 19°C, X-ray diffraction structure determination and analysis by single-wavelength anomalous diffraction at 1.9 A resolution, automated molecular replacement with different search models, and modeling |
| 1.3.1.91 | purified recombinant His-tagged and SeMet-labeled DusC, sitting drop vapour diffusion method, mixing of 200 nl of about 10 mg/ml of protein in 20 mM HEPES, pH 7.6, 1 mM MgCl2, 200 mM KCl, 7 mM 2-mercaptoethanol, and 10% glycerol, with 200 nl of reservoir solution containing 0.1 M Tris, pH 7.9, 0.2 M sodium acetate, and 12% PEG 4000 for the wild-type enzyme and 0.1 M imidazole, pH 8.0, 15% v/v 2-propanol, and 20% v/v glycerol for the SeMet-labeled enzyme, X-ray diffraction structure determination and analysis at 2.1 A resolution |
| 1.3.1.91 | vapor diffusion method, using 1.6 M lithium sulfate and 100 mM HEPES pH 7.5 |
