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EC Number	Crystallization (Commentary)
1.2.1.44	purified recombinant His-tagged enzyme in complex with NADP+, hanging drop, vapor diffusion method, mixing of 20 mg/ml protein in 20 mM Tris base, pH 7.5, 2 mM DTT, and 1 mM NADP+, with an equal volume of reservoir solution , containing 100 mM Bis-Tris, pH 6.5, and 25% w/v PEG 3350, and equilibration against reservoir solution at 4°C, X-ray diffraction structure determination and analysis at 2.35 A resolution, modelling

EC Number

Crystallization (Commentary)

1.2.1.44

purified recombinant His-tagged enzyme in complex with NADP+, hanging drop, vapor diffusion method, mixing of 20 mg/ml protein in 20 mM Tris base, pH 7.5, 2 mM DTT, and 1 mM NADP+, with an equal volume of reservoir solution , containing 100 mM Bis-Tris, pH 6.5, and 25% w/v PEG 3350, and equilibration against reservoir solution at 4°C, X-ray diffraction structure determination and analysis at 2.35 A resolution, modelling

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Release 2023.1 (January 2023)