1.2.1.39 | purified recombinant His-tagged enzyme, sitting drop vapor diffusion method, mixing of 5-7 mg/ml protein in 10 mM Tris, pH 7.5, and 1 mM 2-mercaptoethanol, with 100-200 mM trisodium citrate, pH 8.5, and 12-16% PEG 3350, at 4°C, X-ray diffraction structure determination and analysis at 2.83 A resolution, molecular replacement using ALDH1, PDB ID 1BXS, as a starting model, model building |