EC Number   |
Reference |
|---|
    1.17.1.8 | - |
746195 |
| 1.17.1.8 | crystal structure is determined at 1.92 A |
746463 |
| 1.17.1.8 | crystal structure of the enzyme in the apoform and in complex with its cofactor NADP+, determined at 2.5 A resolution. The crystal belongs to the I4(1)22 space group, and the asymmetric unit of the crystal contains two CgDapB molecules |
745538 |
| 1.17.1.8 | crystal structure of the enzyme in the apoform and in complex with its cofactor NADP+, hanging-drop vapor-diffusion method at 20°C, crystal structure of the enzyme is determined at 2.5 A resolution |
745538 |
| 1.17.1.8 | crystals are grown using the hanging-drop vapour-diffusion method. The crystals belong to the orthorhombic space group P222, with unit-cell parameters a: 80.0, b: 100.8, c: 147.6 A, and contain four molecules in the asymmetric unit |
726634 |
| 1.17.1.8 | hanging drop vapour diffusion method, crystal structure x-ray analysis and binding study with NADPH, NADH, 3-acetyl-NADH, and reduced nicotinamide hypoxanthine dinucleotide phosphate and other pyridine nucleotide derivatives in complex with the enzyme |
390621 |
| 1.17.1.8 | in complex with NADH |
721156 |
| 1.17.1.8 | purified recombinant enzyme, sitting-drop vapour-diffusion method, 100 ml drops of 9-10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 150 mM NaCl with 10 mM 2,6-PDC and 10 mM NADPH are mixed with 100 ml of reservoir solution containing 2.4 M ammonium sulfate, 0.2 M sodium fluoride, and 0.1 M bis-tris propane, pH 7.5, and ethanol added to 10% v/v, 8°C, X-ray diffraction structure determination and analysis at 3.65 A resolution |
710760 |
| 1.17.1.8 | purified recombinant wild-type enzyme in ternary complex with inhibitor 2,6-pyridinedicarboxylate and NADH or NADPH, hanging drop vapour diffusion method, room temperature, 0.003 ml protein solution containing 20 mg/ml enzyme, 2 mM NADH or NADPH, and 30 mM 2,6-pyridinedicarboxylate, mixed with 0.003 ml precipitant solution containing 2.2 M ammonium sulfate, 0.1 M HEPES, pH 7.5, 3% PEG 400, 3 days, X-ray diffraction strcuture determination at 16°C and 2.2 A resolution and analysis |
654674 |
| 1.17.1.8 | R-factor 18.6%, structure model of enzyme complexed with NADPH, analysis of cofactor binding site |
390626 |
