1.14.99.39 | purified recombinant Ny_amoB, amino acids 31-186, sitting drop vapor diffusion method, mixing 0.001 ml of 5 mg/ml protein solution with 0.0035 ml of well solution containing 1 M (NH4)2SO4, 100 mM sodium formate, pH 4.0, and 2% PEG 8000, football shaped crystals appear within 2-3 days, X-ray diffraction structure determination and analysis at 1.8 A resolution, modeling |