EC Number   |
|---|
   1.14.12.12 | crystallized at 8°C using the vapor-diffusion method with a reservoir solution of 2 M (NH4)2SO4 and 2-3% dioxane in 50 mM 2-morpholinoethanesulfonic acid, pH 6.0 |
| 1.14.12.12 | diffraction-quality crystals by hangig-drop method, 2.3 A resolution |
| 1.14.12.12 | hanging drop vapor diffusion method, using 1.9-2.2 M ammonium sulfate, 4-6% (w/v) dioxane, 100 mM MES, pH 5.0-5.8 |
| 1.14.12.12 | hanging drop vapour diffusion method |
| 1.14.12.12 | hanging drop vapour diffusion method using 2-methyl-2,4-pentanediol as a precipitant |
| 1.14.12.12 | modeling of structure with naphthalene bound at the active site. The mononuclear iron is coordinated by amino acid residues His208, His213, and Asp362 and a dioxygen molecule is bound in a side-on fashion. The substrate, naphthalene, is found 2.7 to 3.0 A below the dioxygen molecule and is confined in a small substrate binding site which is surrounded by several hydrophobic residues. The amino acid residues with aromatic side chains, including Phe224, Phe202, Phe352, Trp358, and His295 form a small, but rigid, substrate binding site |
| 1.14.12.12 | vapor diffusion, equal volume of enzyme solution, 30 mg/ml, and reservoir solution, 2 M ammonium sulfate, 2-3% dioxane in 50 mM Mes, pH 6.0, mixed on a cover slip, deep red crystals, refinement of X-ray structure at 2.25 A resolution |
