   1.14.11.6 | purified recombinant full-length or truncated enzyme (unmodified or SeMet-labeled) in apo form, or complexed with 2-oxoglutarate, thymine, 5-hydroxymethyluracil, or 5-formyluracil, drop vapour diffusion method, 16°C, the reservoir solution consists of 0.2 M (NH4)2SO4, 0.1 M MES, pH 6.5, and 30% w/v PEGMME 5000 for the apoenzyme, for the 2-oxoglutarate complexed enzyme of 0.1 M bis-Tris, pH 5.5, and 25% w/v PEG 3,350, and 2-oxoglutarate in a 1:4 ratio protein/2-OG, or for enzyme in complex with other ligands in a 1:4:8 ratio, X-ray diffraction structure determination and analysis at 2.05-2.43 A resolution |
