1.14.11.21 | anaerobically, recombinant isozyme CAS1, in complex with Fe2+, 2-oxoglutarate and deoxyguanidinoproclavaminate, hanging drop vapour diffusion method, protein solution: 20 mg/ml, reservoir solution: 0.1 M Tris, pH 7.5, 2 M ammonium sulfate, 10% glycerol, 20°C, treatment with NO under anaerobic conditions, structure determination and analysis |
639286 |
1.14.11.21 | recombinant isozyme CAS1, hanging drop method, 2 M ammonium sulfate, 0.1 M Tris, 21°C, apoform crystals or in complex with Fe2+, N-alpha-acetyl-L-arginine, and the substrates 2-oxoglutarate and proclavaminate, X-ray diffraction structure determination for native and heavy atom derivatives and analysis |
639281 |