1.1.1.B66 | purified enzyme Bsp5 in complex with D-arginine and coenzyme NADPH, hanging drop vapour diffusion, mixing of 800 nl of 10 mg/m protein in 20 mM HEPES, 50 mM NaCl, pH 7.5, 5 mM D-Arg, and 5 mM NADPH, with 800 nl of reservoir solution containing 20% w/v PEG 3350 and 0.2 M sodium tartrate dibasic, equilibration against 0.05 ml of reservoir solution, room temperature, 10 days, method optimization, X-ray diffraction structure determination and analysis at 1.6 A resolution, molecular replacement, modeling |