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Results 1 - 5 of 5
EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.8-
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.8hanging-drop vapor-diffusion method enzyme/NAD+ complex, enzyme/dihydroxyacetonephosphate complex, enzyme/NAD+/dihydroxyacetonephosphate complex
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.8homology modeling of structure
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.8purified GlpD is concentrated to 8 mg/ml and crystallized at 4°C from a solution containing 0.1 M diammonium hydrogen phosphate, 0.1 M Bicine pH 8.5, and 12% w/v PEG 6000. Structure of the native enzyme, structures of enzyme complexed with substrate analogues phosphoenolpyruvate, glyceric acid 2-phosphate, glyceraldehyde-3-phosphate, and product, dihydroxyacetone phosphate
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.8purified recombinant untagged enzyme, hanging drop vapour diffusion method using a reservoir solution consisting of 12% PEG 8000, 0.1 M Tris-HCl, pH 8.5, 0.3 M MgCl2, X-ray diffraction structure determination and analysis at 2.45 A resolution, molecular replacement and modeling
Results 1 - 5 of 5