EC Number |
---|
1.1.1.44 | - |
1.1.1.44 | 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate, hanging drop vapour diffusion method, using 0.2 M lithium sulfate, 2.2 M ammonium sulfate or 0.2 M ammonium acetate, 2.2 M ammonium sulfate |
1.1.1.44 | complexed with substrate, substrate/NADPH and glucose, sitting drop vapour diffusion method, at 4°C, with 0.12 M diammonium hydrogen citrate buffer (pH 5.0) and 20% (w/v) polyethylene glycol (PEG) 3350 |
1.1.1.44 | complexed with substrate, substrate/NADPH and glucose, sitting drop vapour diffusion method, using 0.1 M sodium citrate dihydrate buffer (pH5.4), 0.5 M ammonium acetate, 6-7% (w/v) PEG 3350, and 17-18% (w/v) PEG 4000 as a reservoir, at 18°C |
1.1.1.44 | crystal structures of recombinant Lactococcus lactis 6-phosphogluconate dehydrogenase in complex with substrate, cofactor, product and inhibitors |
1.1.1.44 | crystals are grown at 22 °C in hanging drops or sitting drops with the vapor diffusion technique. X-ray structures of the enzyme in native form as well as in complex with 6-phosphogluconate or NADP+ at resolutions of 2.8 A, 1.9 A, and 2.9 A. A flexible loop close to the active site rearranges upon binding of 6-phosphogluconate and likely regulates the conformation of the cofactor NADP |
1.1.1.44 | crystals are grown at 22°C in hanging drops with the vapor diffusion technique. X-ray structures of Pf6PGD in native form as well as in complex with 6-phospho-D-gluconate or NADP+ at resolutions of 2.8 A, 1.9 A, and 2.9 A, respectively |
1.1.1.44 | hanging drop vapour diffusion method at 15°C, crystal structure of 6-phosphogluconate dehydrogenase determined at 2.37 Å resolution by molecular replacement |