1.1.1.14 | purified recombinant enyme, hanging drop vapour diffusion method, 22°C, enzyme is complexed with cofactor NAD+ in a molar ratio 1:6, 0.0025 ml protein solution mixed with 0.002 ml well solution containing 0.1 M Tris, pH 8.6, 0.2 M sodium acetate, 10% PEG 3350, and with 0.0005 ml 30% v/v 2-methyl-2,4-pentanediol, 1 week, X-ray diffraction structure determination and analysis at 2.75 A resolution |
654090 |