EC Number   |
Reference |
|---|
    4.1.1.18 | - |
37293, 37294, 37295, 714832 |
| 4.1.1.18 | both isozymes TT1465 and TT1887 |
666850 |
| 4.1.1.18 | cage-like complex of about 3.3 MDa consisting of two LdcI (81 kDa) decamers and up to five RavA (56 kDa) hexamers, hanging drop vapor diffusion method, using |
716768 |
| 4.1.1.18 | hanging drop vapour diffusion method, with 18-28% (w/v) PEG 1000, 100 mM NaCl, 100 mM Tris-HCl pH 8.5, 15% (v/v) glycerol, 5 mM tris(2-carboxyethyl)phospine hydrochloride |
690269 |
| 4.1.1.18 | purified recombinant His6-tagged enzyme, mixing 0.001 ml of 65 mg/ml protein in 40 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution, containing 28% w/v PEG 400, 0.1 M sodium citrate tribasic-citric acid, pH 6.0, and 0.2 M MgCl2, and equilibration against 0.5 ml of reservoir solution, 20°C, the apo-form II crystals of SrLDC are crystallized using a reservoir solution containing 1.15 M sodium citrate and 0.1 M sodium cacodylate, pH 5.0. The crystals of SrLDC complexed with PLP/cadaverine are crystallized using 50% PEG 200, 0.1 M sodium phosphate, pH 4.2, 0.2 M sodium chloride, and 5 mM PLP, followed by soaking in 10 mM L-lysine solution for 30 min, X-ray diffraction structure determination and analysis at 2.0-2.9 A resolution, molecular replacement with the structure of L/ODC from Vibrio vulnificus (VvL/ODC, PDB ID 2PLK) as search model, model building |
749097 |
| 4.1.1.18 | purified SrLDCA225C/T302C mutant, hanging drop vapour diffusion method, mixing of 0.001 ml of 65 mg/ml protein in 40 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution containing 1.4 M ammonium sulfate, 0.1 M sodium cacodylate, pH 6.5, and 0.2 M sodium chloride, and equilibration against 0.5 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 1.8 A resolution, molecular replacement using the structure of refined SrLDC as a model, model building |
749101 |
