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wild-type and mutant caspase-2 enzymes complexed with peptide aldehyde inhibitors, incubation of 3 mg/ml protein with 1-5 mM peptide aldehyde inhibitor in DMSO at room temperature for 2 h, hanging drop vapour diffusion method, mixing of 0.002 ml of protein complex solution with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.0, 15% PEG 3350, 3 mM DTT, X-ray diffraction structure determination and analysis at 1.5-2.4 A resolution, molecular replacement
Results 1 - 2 of 2