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EC Number
purified recombinant mutant TVMV protease K65A/K67A/C151A in complex with a canonical peptide substrate RETVRFQSD, mixing of protein solution with 10 mg/ml protein with a fivefold molar excess of the peptide substrate RETVRFQSD, crystallization from a solution consisting of 0.2 M potassium formate and 20% PEG 3350, space group P212121, X-ray diffraction structure determination and analysis at 1.7 A resolution, the 20-residue C-terminus of TVMV protease is disordered, molecular replacement, using the crystal structure of TEV protease, PDB code 1Q31
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