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EC Number
enzyme in complex with inhibitors, hanging drop vapor diffusion method, mixing of 10 mg/ml protein in 20 mM HEPES with 5 mm ligands, with reservoir solution containing 0.1 M sodium acetate, pH 4.0, 0.2 M ammonium acetate, 3% 2-propanol, and 15% PEG 10000, X-ray diffraction structure determination and analysis, molecular replacement
mono N-glycosylated forms of soluble enzyme ecto-domain (residues 32-278) and catalytic residue mutant Glu218Gln, in apo state or bound to 2'-fluorinated NAD+ derivatives aFNAD or rFNAD, hanging drop vapour diffusion method, from 20-30% PEG 4000, 50-250 mM ammonium sulfate and 100 mM sodium cacodylate, sodium acetate or MES, pH 6.0-6.5, room temperature, soaking of crystals in 1-3 mM ligand solution, X-ray diffraction structure determination and analysis at 1.8 A resolution, molecular replacement
Results 1 - 2 of 2