EC Number |
---|
2.7.4.25 | enzyme in complex with CDP |
2.7.4.25 | hanging drop vapor diffusion method, using 0.4 M ammonium sulfate in 50 mM Tris-HCl buffer (pH 7.4), at 20°C |
2.7.4.25 | hanging drop vapor diffusion technique |
2.7.4.25 | in complex with substrates CMP, dCMP, ara-CMP and 2,3-dideoxy-CMP, hanging drop vapor diffusion method, using ammonium sulfate in a 50 mM Tris-HCl buffer (pH 7.4), at 20°C |
2.7.4.25 | mutant enzyme R188M is crystallized either alone or in complex with dCMP, hanging drop vapor diffusion method, using ammonium sulfate as a precipitant (1.3 M in the case of enzyme alone, and 1.7 M for the R188M-dCMP complex) |
2.7.4.25 | of the R188M mutant alone or with dCMP |
2.7.4.25 | purified recombinant mutant R188M free or in complex with CMP, X-ray diffraction structure determination and analysis at 1.9 A and 2.8 A resolution, respectively |