EC Number   |
Reference  |
|---|
    2.3.1.47 | pyridoxal-5'-phosphate-bound form, pH 7.5, precipitation with polyethylene glycol, 0.2 M (NH4)2SO4 , x-ray diffraction structure analysis |
487055 |
| 2.3.1.47 | pH 8.5 |
487056 |
| 2.3.1.47 | Mg2+ added for structure analysis of the holoenzyme |
487058 |
| 2.3.1.47 | pH 8.5-8.9, ammonium sulfate precipitation, apoenzyme, X-ray diffraction structure analysis with and without bound pyridoxyl-5'-phosphate, model, active site residues |
487057, 487058 |
| 2.3.1.47 | enzyme in complex with inhibitor trifluoroalanine, soaking of holo-enzyme crystals in a solution containing 10 mM L-trifluoroalanine and 10 mM pyridoxal-5'-phosphate in crystallization buffer containing 1.6 M ammonium sulfate and 200 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane at pH 8.5, and rapidly frozen in liquid nitrogen, X-ray diffraction structure determination and analysis at 1.99 A resolution |
676274 |
| 2.3.1.47 | purified recombinant enzyme, hanging-drop vapour diffusion, mixing of 0.002 ml of 5 mg/ml protein in 20 mM Tris-HCl pH 7.9, 100 mM NaCl, 2 mM DTT, with 0.002 ml of reservoir solution containing 150 mM DL-malic acid pH 7.4, 100 mM NaCl, 25% PEG 3350, equilibrqtion against 0.2 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.3 A resolution |
735394 |
| 2.3.1.47 | the crystal structure of MsBioF is solved by molecular replacement using the structure of EcAONS, PDB code 1BS0, as the search model |
736245 |
