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Results 1 - 7 of 7
EC Number
Crystallization
Reference
enzyme in complex with inhibitor trifluoroalanine, soaking of holo-enzyme crystals in a solution containing 10 mM L-trifluoroalanine and 10 mM pyridoxal-5'-phosphate in crystallization buffer containing 1.6 M ammonium sulfate and 200 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane at pH 8.5, and rapidly frozen in liquid nitrogen, X-ray diffraction structure determination and analysis at 1.99 A resolution
Mg2+ added for structure analysis of the holoenzyme; pH 8.5-8.9, ammonium sulfate precipitation, apoenzyme, X-ray diffraction structure analysis with and without bound pyridoxyl-5'-phosphate, model, active site residues
pH 8.5
pH 8.5-8.9, ammonium sulfate precipitation, apoenzyme, X-ray diffraction structure analysis with and without bound pyridoxyl-5'-phosphate, model, active site residues
purified recombinant enzyme, hanging-drop vapour diffusion, mixing of 0.002 ml of 5 mg/ml protein in 20 mM Tris-HCl pH 7.9, 100 mM NaCl, 2 mM DTT, with 0.002 ml of reservoir solution containing 150 mM DL-malic acid pH 7.4, 100 mM NaCl, 25% PEG 3350, equilibrqtion against 0.2 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.3 A resolution
pyridoxal-5'-phosphate-bound form, pH 7.5, precipitation with polyethylene glycol, 0.2 M (NH4)2SO4 , x-ray diffraction structure analysis
the crystal structure of MsBioF is solved by molecular replacement using the structure of EcAONS, PDB code 1BS0, as the search model
Results 1 - 7 of 7