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Results 1 - 9 of 9
EC Number
Crystallization
Reference
crystal structure analysis, PDB ID 3CHX, structure modeling
enzyme pMMO crystal structure analysis, PDB ID 3RGB
hanging drop vapor diffusion method
heterotrimer of three subunits, pmoA, pmoB, and pmoC, with a solvent-exposed domain above the trans-membrane domain, which consists of alpha-helices
purified native enzyme, sitting drop vapour diffusion method, mixing of 0.0025 ml of 20 mg/ml pMMO protein, and 0.0005 ml of 85 mM n-octyl beta-D-thiomaltoside with 0.002 ml of 10% PEG 3000, 200 mM magnesium formate dihydrate, 100 mM sodium cacodylate trihydrate, pH 6.5, at room temperature, 1-4 weeks, for the complex structure, the crystals are soaked in Zn2+ solution, X-ray diffraction structure determination and analysis at 2.6 A resolution
sitting drop vapor diffusion method, using 0.1 M MES, pH 6.5, 18% (w/v) PEG 750 MME, 0.03 M glycyl glycine, 0.01 M ZnSO4, at 22°C
to 2.8 A resolution
to 3.9 A resolution
Results 1 - 9 of 9