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EC Number
Crystallization
Reference
hanging drop vapor diffusion method
the resonance Raman modes of the oxidized and two-electron reduced forms of Npx are related to very tight flavin-protein interactions maintaining a nearly planar conformation of the isoalloxazine tricycle, a low level of H-bonding at the N1/N5 and O2/O4 sites, and a strong H-bond at N3H. They indicate minimal changes in FAD structure and environment upon either NAD(H) binding or reduction of the sulfinic redox center
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