EC Number   |
Cofactor |
Reference |
|---|
    4.1.99.1 | pyridoxal 5'-phosphate |
- |
37341, 37344, 677276, 678747, 681216, 681998, 691648, 701449, 702931, 703486, 704093, 704722, 704847, 705578, 705579, 705580, 706000, 713955, 715733, 726995, 728262, 728650 |
| 4.1.99.1 | pyridoxal 5'-phosphate |
binds 1 mol of pyridoxal 5'-phosphate per subunit |
37335 |
| 4.1.99.1 | pyridoxal 5'-phosphate |
contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme |
37346, 37350, 37354 |
| 4.1.99.1 | pyridoxal 5'-phosphate |
conversion of apoenzyme into the active holoenzyme is attained at 30°C in Tris-HCl buffer, pH 8.0, containing pyridoxal 5'-phosphate and K+, no conversion occurs at 5°C |
37336 |
| 4.1.99.1 | pyridoxal 5'-phosphate |
covalent binding required to activate the enzyme |
671054 |
| 4.1.99.1 | pyridoxal 5'-phosphate |
dependent on |
37334, 37335, 37336, 37347, 37349, 37350, 37352, 37354, 37356, 671054, 746632, 746654, 746875, 746909, 747167, 747414 |
| 4.1.99.1 | pyridoxal 5'-phosphate |
dependent on, monovalent cations (K+ or NH4+) are required for the binding of pyridoxal 5'-phosphate to a lysine residue in the active site, leading to the functionally active form. Each monomer binds one molecule of pyridoxal 5'-phosphate, which forms an aldimine bond to the Lys270 residue in the active site |
746651 |
| 4.1.99.1 | pyridoxal 5'-phosphate |
dependent on, residue Arg103 plays an important role in orientation of the cofactor pyridoxal 5'-phosphate |
746778 |
| 4.1.99.1 | pyridoxal 5'-phosphate |
dependent on, the active site residues involved in cofactor binding are highly conserved for enzyme TIL |
747245 |
| 4.1.99.1 | pyridoxal 5'-phosphate |
dependent on, the activity of TPase can be modulated by the concentration of pyridoxal 5'-phosphate |
746742 |
