EC Number   |
Cofactor |
Reference |
|---|
    4.1.1.15 | Calmodulin |
- |
713972, 716552 |
| 4.1.1.15 | Calmodulin |
a unique feature of plant GAD is the presence of a calmodulin (CaM)-binding domain at its C-terminus. In plants, transient elevation of cytosolic Ca2+ in response to different types of stress is responsible for GAD activation via CaM |
747173 |
| 4.1.1.15 | Calmodulin |
Ca2+/calmodulin-dependent enzyme with calmodulin binding activity |
3993 |
| 4.1.1.15 | Calmodulin |
GAD can be regulated in vivo by Ca2+/calmodulin atpH 5.8. The addition of bovine calmodulin and Ca2+ to the reaction mix causes an activation of GAD activity of 32% |
716650 |
| 4.1.1.15 | Calmodulin |
plus Ca2+, at pH 5.8, activates |
3985 |
| 4.1.1.15 | Calmodulin |
stimulation be Ca2+ plus calmodulin, no stimulation by Ca2+ or calmodulin alone |
3987 |
| 4.1.1.15 | more |
the cofactor of ScGAD is verified to be either pyridoxal 5'-phosphate (PLP) or pyridoxal. The optimal concentration of either cofactor is 50 mg/l. GAD requires a coenzyme for activity while inactive apo-GAD forms a GAD-PLP complex (holo-GAD) to obtain its activity |
746797 |
| 4.1.1.15 | pyridoxal 5'-phosphate |
- |
664478, 682084, 696808, 696820, 696849, 700110, 700472, 700597, 704740, 705157, 705391, 705392, 705636, 705638, 713797, 713835, 714584, 714866, 715832, 715982, 716552, 716650, 717007, 727381, 727641, 728005, 728019, 746711, 746715, 746729, 746797, 747173, 747308, 747356, 747486, 747609, 747785, 747999, 748276, 748285, 748355, 748575, 748583, 748691, 748766, 749087, 749426 |
| 4.1.1.15 | pyridoxal 5'-phosphate |
20% activity in absence |
679326 |
| 4.1.1.15 | pyridoxal 5'-phosphate |
absolutely required |
699880 |
