EC Number   |
Cofactor |
Reference |
|---|
    1.3.5.5 | decyl-plastoquinone |
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially |
712210 |
| 1.3.5.5 | FAD |
PDS requires plastoquinone as a directly interacting co-substrate to reoxidize the enzyme-bound FADred formed upon desaturation |
763616 |
| 1.3.5.5 | FAD |
sole protein-bound redox-cofactor |
743580 |
| 1.3.5.5 | FAD |
the deduced amino acid sequence of wheat phytoene desaturase reveals a typical pyridine dinucleotide binding motif generally associated with the binding of FAD |
712989 |
| 1.3.5.5 | more |
5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole, 2,5-dibromo-3-methyl-6-isopropyl-l,4-benzoquinone, duroquinon are used as artificial electron acceptor |
7910 |
| 1.3.5.5 | more |
the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone |
712210 |
| 1.3.5.5 | more |
the soluble form of the enzyme is able to associate on to/into protein-free liposomal membranes made from chromoplast lipids, thereby gaining activity by binding added FAD. Once bound to membranes, activated phytoene desaturase works independently of any added FAD, employing membrane-bound electron acceptors. FAD exerts no positive effect on the membrane-association process, its role is confined to enzymatic activation |
713288 |
| 1.3.5.5 | plastoquinone |
- |
713208 |
| 1.3.5.5 | plastoquinone |
decyl-plastoquinone or decyl-ubiquinone is used in the study because the natural C45-plastoquinone is not available commercially. The affinity of the enzyme for plastoquinone is higher than for NADP+ |
712210 |
| 1.3.5.5 | plastoquinone |
plastoquinone is an essential component of phytoene desaturation |
713273 |
