EC Number   |
Cofactor |
Reference |
|---|
  1.17.1.1 | more |
0.37 SH groups per molecule of enzyme E3, in the absence of SDS and 1 SH group per molecule of enzyme E3 in the presence of SDS, the single SH group in enzyme E3 is essential for activity |
437992, 437994 |
| 1.17.1.1 | more |
3.8 SH groups per molecule of enzyme E1, in the presence and absence of SDS |
437992 |
| 1.17.1.1 | more |
4.3 SH per mol of enzyme E1 in the presence of SDS, calculated using a molecular weight of 61000 Da |
437994 |
| 1.17.1.1 | more |
derivatives of vitamin B6 such as pyridoxine, pyridoxine 5'-phosphate, pyridoxal, pyridoxal 5'-phosphate, pyridoxamine and pyridoxamine 5'-phosphate have stimulatory effect on the reaction, indicating that the amino group and the phosphate group are necessary for the reaction |
437992 |
| 1.17.1.1 | NAD+ |
- |
745601 |
| 1.17.1.1 | NADH |
- |
745601 |
| 1.17.1.1 | NADH |
the second substrate of the reaction, the hydrogen donor |
437992 |
| 1.17.1.1 | NADPH |
- |
437993 |
| 1.17.1.1 | NADPH |
the second substrate of the reaction, the hydrogen donor |
437992 |
| 1.17.1.1 | pyridoxamine 5'-phosphate |
bound to enzyme E1 through an ionic interaction with a positive charge on the surface of the enzyme, the cofactor is needed for the binding of the substrate to the enzyme |
437992, 437994 |
