EC Number   |
Cofactor |
Reference |
|---|
  1.21.98.3 | Cobalamin |
- |
737606 |
| 1.21.98.3 | S-adenosyl-L-methionine |
- |
744228 |
| 1.21.98.3 | [4Fe-4S]-center |
BchE sequence displays the conserved CXXXCXXC sequence essential for 4Fe-4S cluster formation and an active 4Fe-4S cluster is present in the protein. Isolated protein is yellow-brown in colour with an absorption peak around 410 nm |
738567 |
| 1.21.98.3 | Cobalamin |
dependent on, ligated via Asp248, Glu249, Leu29, Thr71, Val97 |
764107 |
| 1.21.98.3 | S-adenosyl-L-methionine |
dependent on, ligated via Phe210, Glu308 and Lys320 |
764107 |
| 1.21.98.3 | [4Fe-4S] cluster |
ligated viaCys204, Cys208, Cys211 |
764107 |
| 1.21.98.3 | more |
membrane-localized BchE requires an additional, heat-sensitive cytosolic cobalamin component for activity, BchE catalysis is not sustained in chimeric experiments when a cytosolic extract from Escherichia coli is used as a substitute |
764107 |
| 1.21.98.3 | Cobalamin |
vitamin B12-requiring mutants of the bluE and bluB genes of Rhodobacter capsulatus, grown without B12, accumulate Mg-protoporphyrin monomethyl ester and its 3-vinyl-8-ethyl derivative. Cyclase activity in the B12-dependent mutants requires vitamin B12 but not protein synthesis |
739563 |
