EC Number   |
Cofactor  |
Reference |
|---|
   1.21.99.5 | corrinoid |
one mol of dehalogenase contains 1.0 mol of corrinoid |
349700 |
| 1.21.99.5 | corrinoid |
presence of a corrinoid in the cob(II)alamin form |
349700 |
| 1.21.99.5 | corrinoid |
vitamin B12 |
349700, 349707 |
| 1.21.99.5 | menaquinone |
membrane-bound menaquinone |
349702 |
| 1.21.99.5 | more |
anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12 |
727374 |
| 1.21.99.5 | more |
no effect by NADH |
349709 |
| 1.21.99.5 | vitamin B12 |
- |
349700, 349701, 349703, 349707 |
| 1.21.99.5 | vitamin B12 |
corrinoid cofactor is norpseudo-B12, adenine instead of 5,6-dimethylbenzimidazole serves as lower ligand base of the central cobalt ion |
727374 |
| 1.21.99.5 | vitamin B12 |
PceA dehalogenase harbors either norpseudo-B12 as cofactor when Sulfurospirillum multivorans has been cultivated in the absence - or nor-B12 when Sulfurospirillum multivorans has been cultivated in its presence of 4,6-dimethylbenzimidazole. Dehalogenation rates with norpseudo-B12 reductive dehalogenase are faster than with nor-B12 |
740384 |
