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EC Number
Cofactor
Commentary
Reference
1.21.99.5
corrinoid
dicyano-cob(III)alamin
349706
1.21.99.5
corrinoid
corrinoid probably noncavalently bound to enzyme
349708
1.21.99.5
corrinoid
Co2+ corrinoid
349709
1.21.99.5
more
no effect by NADH
349709
1.21.99.5
Cobalamin
-
657587
1.21.99.5
corrinoid
cofactor is absent in strain N and therefore the strain is unable to dechlorinate tetrachloroethene
657689
1.21.99.5
more
anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12
727374
1.21.99.5
vitamin B12
corrinoid cofactor is norpseudo-B12, adenine instead of 5,6-dimethylbenzimidazole serves as lower ligand base of the central cobalt ion
727374
1.21.99.5
vitamin B12
PceA dehalogenase harbors either norpseudo-B12 as cofactor when Sulfurospirillum multivorans has been cultivated in the absence - or nor-B12 when Sulfurospirillum multivorans has been cultivated in its presence of 4,6-dimethylbenzimidazole. Dehalogenation rates with norpseudo-B12 reductive dehalogenase are faster than with nor-B12
740384
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