EC Number |
pH Stability |
pH Stability Maximum |
Reference |
---|
3.4.22.2 | 1 |
2 |
after 60 min of incubation the immobilized enzyme retains about 98% of its residual activity |
753834 |
3.4.22.2 | 1 |
2 |
half life after 60 storage: below 5% free papain, 40% immobilized papain |
733073 |
3.4.22.2 | 2 |
- |
residual activity: 10% free papain, 40% immobilized papain |
733073 |
3.4.22.2 | 2 |
- |
the activity of papain is reduced to 35% at pH 2 and in the absence of detergents, at pH 2 papain exhibits a substantial amount of secondary structure and is relatively less denatured compared with 6 M guanidine hydrochloride but loses the persistent tertiary contacts of the native state |
678740 |
3.4.22.2 | 2 |
7 |
25°C, stable at pH 7.0, acid unfolding at pH 2.0 |
667306 |
3.4.22.2 | 3 |
- |
after 60 min of incubation the immobilized enzyme retains about 53% of its residual activity |
753834 |
3.4.22.2 | 3 |
- |
half life after 60 storage: below 5% free papain, 40% immobilized papain |
733073 |
3.4.22.2 | 4 |
- |
rapid and irreversible inactivation, at elevated temperatures |
95663 |
3.4.22.2 | 4 |
10 |
stable |
95685 |
3.4.22.2 | 4 |
10 |
the enzyme retains more than 80% of initial activity after 30 min incubation at pH 3.0-10.0 |
752461 |