    2.3.1.46 | 5.4 |
7.5 |
25°C, histidine-tagged enzyme, 0-50 mM acetic acid: 0 mM = pH 7.5, 10 mM = 7.0, 20 mM = 6.6, 30 mM = 6.2, 40 mM = 5.8, 50 mM = 5.4, the recombinant enzyme shows reduced activity at 25°C without great change at about 200 micromol/min/mg protein, about 3.5times higher activity at 40°C |
701784 |
| 2.3.1.46 | 5.4 |
7.5 |
25°C, histidine-tagged enzymes, 0-50 mM acetic acid: 0 mM = pH 7.5, 10 mM = 7.0, 20 mM = 6.6, 30 mM = 6.2, 40 mM = 5.8, 50 mM = 5.4, sharp decrease of wild-type enzyme activity above 30 mM acetic acid, no activity at 50 mM, without acid about 2900 micromol/min/mg protein, the mutant strain 333 (S61T/E213V/I229T/N267D/N271K) enzyme retains almost 50% activity at 50 mM acetic acid, activity decreases above 30 mM from about 2000 micromol/min/mg protein, the mutants N267D and I229T do not greatly change their activity, about 2000-1800 micromol/min/mg protein, and about 2500-2000 micromol/min/mg protein, respectively |
701784 |
