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EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Reference
3.4.22.6
-999
-
pH value of the reaction medium plays a significant role in maintaining the stability and catalytic activity of the enzyme
733046
3.4.22.6
4.91
-
assay at. Chymopapain shows the best activity and enantioselectivity in the presence of phosphate buffer (pH 4.91, buffer/MeCN:0.12, v/v), which give the product in yield of 45% with 72:28 (anti/syn ratio) and 78% ee (for anti isomer)
733046
3.4.22.6
5.5
8
benzoyl-L-argininamide
81562
3.4.22.6
6.2
-
assay at
695547
,
733086
3.4.22.6
7
-
hemoglobin digestion
81562
3.4.22.6
7
9
casein digestion
81562
3.4.22.6
7.2
-
casein or hemoglobin digestion
81564
3.4.22.6
7.2
-
enzyme is dissolved in phosphate buffer and used with a suspension of lysosomes in 0.154 mol/l sodium chloride
695532
3.4.22.6
7.4
-
assay at
695547
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