EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    6.3.2.3 | -999 |
- |
more |
loopless mutant with a 930fold decrease in turnover number |
1219 |
| 6.3.2.3 | -999 |
- |
more |
turnover number is fatally reduced in the P227V/G240V mutant |
1218 |
| 6.3.2.3 | -999 |
- |
more |
turnover-numbers of wild-type and mutant enzymes |
1209 |
| 6.3.2.3 | 0.003 |
- |
gamma-L-Glu-L-Cys |
mutant E144K and mutant N146D, pH 8.2, 37°C |
652539 |
| 6.3.2.3 | 0.007 |
- |
gamma-L-Glu-L-Cys |
mutant N146A, pH 8.2, 37°C |
652539 |
| 6.3.2.3 | 0.008 |
- |
gamma-L-Glu-L-Cys |
mutant K364A, pH 8.2, 37°C |
652539 |
| 6.3.2.3 | 0.011 |
- |
gamma-Glu-L-Cys |
mutant enzyme K456M, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase |
674814 |
| 6.3.2.3 | 0.015 |
- |
gamma-L-Glu-L-Cys |
mutant K364E, pH 8.2, 37°C |
652539 |
| 6.3.2.3 | 0.052 |
- |
Gly |
mutant G370V, 37°C, pH not specified in the publication |
714035 |
| 6.3.2.3 | 0.059 |
- |
gamma-Glu-L-Cys |
mutant enzyme R132K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase |
674814 |
