EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
5.4.99.5 | -999 |
- |
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide |
turnover of mutant H189N enzyme is lower than 0.0025 per second |
390552 |
5.4.99.5 | -999 |
- |
more |
CM spezific activity in Escherichia coli extracts: Control (M. Tuberculosis) Sp act (U/mg) 0.889 Cloned extract/control 1 AroQMt (Rv0948c) Sp act (U/mg) 127.38 Cloned extract/control 143.28 *AroQMt (Rv1885c) Sp act (U/mg) 94.23 Cloned extract/control 105.9. CM specific activity in Escherichia coli cells expressing either the AroQMt or the *AroQMt protein is determined to be, repectively, 143- and 106-fold higher than the enzyme activity obtained for Escherichia coli cells carrying the pET-23a(+) expression vector |
674346 |
5.4.99.5 | -999 |
- |
more |
R90Cit 10E4-fold decrease in the catalytic activity of kcat. R90K 10E4-fold decrease in the catalytic activity of kcat/Km is obtained |
675611 |
5.4.99.5 | -999 |
- |
more |
The kcat of the active complementations (position 7, 32, 35, 48, 81 and 85) is shown |
672268 |
5.4.99.5 | -999 |
- |
more |
turnover of mutant H189N enzyme is lower than 0.0025 per second |
390552 |
5.4.99.5 | 0.0003 |
- |
chorismate |
30°C, pH 7, mutant R90G |
652061 |
5.4.99.5 | 0.00983 |
- |
chorismate |
mutant enzyme Lys39Arg |
3568 |
5.4.99.5 | 0.042 |
- |
chorismate |
mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C |
690920 |
5.4.99.5 | 0.0492 |
- |
chorismate |
mutant enzyme Lys39Asn |
3568 |
5.4.99.5 | 0.11 |
- |
chorismate |
mutant enzyme G86A |
703449 |