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EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Reference
5.4.99.2	0.003	-	(R)-2-methylmalonyl-CoA	37°C, mutant H610N	652252
5.4.99.2	0.012	-	(R)-methylmalonyl-CoA	recombinant His-tagged mutant E392D, pH 8.5, 25°C	727036
5.4.99.2	0.024	-	(R)-2-methylmalonyl-CoA	37°C, mutant H610A	652252
5.4.99.2	0.038	-	succinyl-CoA	25°C, mutant H244A	650005
5.4.99.2	0.25	-	(R)-methylmalonyl-CoA	recombinant His-tagged mutant E392Q, pH 8.5, 25°C	727036
5.4.99.2	0.32	-	(R)-methylmalonyl-CoA	recombinant His-tagged mutant E392A, pH 8.5, 25°C	727036
5.4.99.2	0.33	-	(R)-2-methylmalonyl-CoA	mutant Y243A, pH 7.0, 37°C	678179
5.4.99.2	0.66	-	succinyl-CoA	25°C, mutant H244Q	650005
5.4.99.2	3.95	-	(R)-methylmalonyl-CoA	recombinant His-tagged wild-type enzyme, pH 8.5, 25°C	727036
5.4.99.2	6.08	-	succinyl-CoA	25°C, mutant H244Q	650005

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Release 2023.1 (January 2023)