EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    5.3.99.2 | 0.06 |
- |
prostaglandin H2 |
mutant enzyme L79A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C |
714938 |
| 5.3.99.2 | 0.37 |
- |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate |
- |
649547 |
| 5.3.99.2 | 0.5 |
- |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate |
in the presence of 1 M urea |
649547 |
| 5.3.99.2 | 0.63 |
- |
prostaglandin H2 |
mutant enzyme M64A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C |
714938 |
| 5.3.99.2 | 0.81 |
- |
prostaglandin H2 |
mutant enzyme L131A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C |
714938 |
| 5.3.99.2 | 0.87 |
- |
prostaglandin H2 |
mutant enzyme F83A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C |
714938 |
| 5.3.99.2 | 0.95 |
- |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate |
in the presence of 500 mM guanidinium hydrochloride |
649547 |
| 5.3.99.2 | 2 |
- |
glutathione |
conjugation of glutathione to 1-chloro-2,4-dinitrobenzene |
652044 |
| 5.3.99.2 | 2.23 |
- |
prostaglandin H2 |
mutant enzyme S45A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C |
714938 |
| 5.3.99.2 | 2.24 |
- |
prostaglandin H2 |
mutant enzyme M94A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C |
714938 |
