EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
4.2.2.2 | 0.03 |
0.55 |
polygalacturonic acid |
pH 10.5, 30°C, mutant enzyme chimeric enzyme composed of Ala1 to Tyr105 of Pel-15 in the N-terminal regions, Asp133 to Arg 159 of pectate lyase B from Fusarium solani in the internal regions, and Gln133 to Tyr197 of Pel-15 in the C-terminal regions |
651067 |
4.2.2.2 | 6.6 |
- |
digalacturonic acid |
- |
681783 |
4.2.2.2 | 7.48 |
- |
polygalacturonic acid |
pH 10.5, 30°C, mutant enzyme chimeric enzyme composed of Ala1 to Tyr105 of Pel-15 in the N-terminal regions, Asp133 to Arg 159 of pectate lyase B from Fusarium solani in the internal regions, and Gln133 to Tyr197 of Pel-15 in the C-terminal regions |
651067 |
4.2.2.2 | 21 |
- |
Pectin |
- |
681783 |
4.2.2.2 | 22 |
- |
89% esterified citrus pectin |
37°C |
649703 |
4.2.2.2 | 42.2 |
- |
polygalacturonic acid |
pH 10.5, 30°C, mutant enzyme K89A |
651067 |
4.2.2.2 | 43.2 |
- |
polygalacturonic acid |
pH 10.5, 30°C, mutant enzyme D84N |
651067 |
4.2.2.2 | 65.95 |
- |
trigalacturonic acid |
- |
681783 |
4.2.2.2 | 72.9 |
- |
polygalacturonic acid |
pH 10.5, 30°C, mutant enzyme D126N |
651067 |
4.2.2.2 | 101 |
- |
polygalacturonic acid |
pH 10.5, 30°C, mutant enzyme H66A |
651067 |