EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    3.6.1.23 | 0.0003 |
- |
dUTP |
D90N mutant enzyme, pH 7.8, room temperature |
656250 |
| 3.6.1.23 | 0.00089 |
- |
dUTP |
mutant enzyme G143STOP, at pH 7.5 and 20°C |
757063 |
| 3.6.1.23 | 0.0035 |
- |
dUTP |
mutant enzyme T138STOP, at pH 7.5 and 20°C |
757063 |
| 3.6.1.23 | 0.0045 |
- |
dUTP |
mutant enzyme R140K/H145W, at pH 7.5 and 20°C |
757063 |
| 3.6.1.23 | 0.007 |
- |
dUTP |
mutant enzyme D49N/F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C |
712021 |
| 3.6.1.23 | 0.024 |
- |
dUTP |
mutant enzyme D28N/H145W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C |
712021 |
| 3.6.1.23 | 0.04 |
- |
dUTP |
mutant enzyme D131N, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C |
712381 |
| 3.6.1.23 | 0.04 |
- |
dUTP |
mutant enzyme D131S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C |
712381 |
| 3.6.1.23 | 0.053 |
- |
dUTP |
mutant enzyme R161K, at pH 7.5 and 20°C |
757196 |
| 3.6.1.23 | 0.06 |
- |
dUTP |
mutant enzyme R268A, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C |
712381 |
