EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    3.5.99.2 | 0.001 |
- |
4-amino-5-aminomethyl-2-methylpyrimidine |
mutant enzyme F47Y, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C |
711977 |
| 3.5.99.2 | 0.0017 |
- |
thiamine |
in 100 mM Tris-HCl, pH 7.5, at 37°C |
713350 |
| 3.5.99.2 | 0.003 |
- |
4-amino-5-aminomethyl-2-methylpyrimidine |
wild type enzyme, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C |
711977 |
| 3.5.99.2 | 0.00407 |
- |
thiamine |
wild type enzyme, at pH 7.5 and 37°C |
733003 |
| 3.5.99.2 | 0.0068 |
- |
thiamine |
mutant enzyme D111A/K115A, at pH 7.5 and 37°C |
733003 |
| 3.5.99.2 | 4.83 |
- |
thiamine |
- |
246758 |
