EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   3.4.22.59 | -999 |
- |
more |
- |
647471 |
| 3.4.22.59 | 0.015 |
- |
N-acetyl-VEID-7-amido-4-methylcoumarin |
mutant enzyme E244A/H287A, in 100 mM HEPES, pH 7.5, 10% (w/v) sucrose, 0.1% (w/v) CHAPS, 20 mM TCEP, and 120 mM NaCl, at 37°C |
732072 |
| 3.4.22.59 | 0.05 |
- |
Ac-Val-Glu-Ile-Asp-7-amido-4-trifluoromethylcoumarin |
37°C, pH 7.2, mutant enzyme L200A |
755524 |
| 3.4.22.59 | 0.051 |
- |
N-acetyl-VEID-7-amido-4-methylcoumarin |
mutant enzyme E244A, in 100 mM HEPES, pH 7.5, 10% (w/v) sucrose, 0.1% (w/v) CHAPS, 20 mM TCEP, and 120 mM NaCl, at 37°C |
732072 |
| 3.4.22.59 | 0.309 |
- |
N-acetyl-VEID-7-amido-4-methylcoumarin |
mutant enzyme H287A, in 100 mM HEPES, pH 7.5, 10% (w/v) sucrose, 0.1% (w/v) CHAPS, 20 mM TCEP, and 120 mM NaCl, at 37°C |
732072 |
| 3.4.22.59 | 0.371 |
- |
N-acetyl-VEID-7-amido-4-methylcoumarin |
mutant enzyme K36A, in 100 mM HEPES, pH 7.5, 10% (w/v) sucrose, 0.1% (w/v) CHAPS, 20 mM TCEP, and 120 mM NaCl, at 37°C |
732072 |
| 3.4.22.59 | 0.7 |
- |
N-acetyl-VEID-7-amido-4-methylcoumarin |
wild type enzyme, in 100 mM HEPES, pH 7.5, 10% (w/v) sucrose, 0.1% (w/v) CHAPS, 20 mM TCEP, and 120 mM NaCl, at 37°C |
732072 |
| 3.4.22.59 | 1 |
- |
N-acetyl-Ile-Val-Leu-Asp-7-amido-4-methylcoumarin |
- |
707434 |
| 3.4.22.59 | 1.39 |
- |
N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin |
pH 7.5, 37°C, mutant enzyme R44K |
754186 |
| 3.4.22.59 | 1.59 |
- |
N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin |
pH 7.5, 37°, wild-type enzyme |
754186 |
