EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.1.4.52 | 0.000011 |
- |
cyclic di-3',5'-guanylate |
pH 8.0, 23°C, recombinant mutant E352Q |
692857 |
3.1.4.52 | 0.000011 |
- |
cyclic-di-GMP |
mutant enzyme E352Q, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2 |
692857 |
3.1.4.52 | 0.000022 |
- |
cyclic di-3',5'-guanylate |
pH 8.0, 23°C, recombinant mutant E352D |
692857 |
3.1.4.52 | 0.000022 |
- |
cyclic-di-GMP |
mutant enzyme E352D, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2 |
692857 |
3.1.4.52 | 0.00012 |
- |
5'-phosphoguanylyl(3'->5')guanosine |
pH 8.0, 30°C, recombinant enzyme |
730749 |
3.1.4.52 | 0.00015 |
- |
cyclic di-3',5'-guanylate |
pH 8.0, 30°C, recombinant enzyme |
730749 |
3.1.4.52 | 0.0002 |
- |
5'-phosphoguanylyl(3'->5')guanosine |
pH 8.0, 30°C, recombinant wild-type enzyme |
730749 |
3.1.4.52 | 0.0002 |
- |
cyclic di-3',5'-guanylate |
pH 8.0, 30°C, recombinant wild-type enzyme |
730749 |
3.1.4.52 | 0.0006 |
- |
cyclic di-3',5'-guanylate |
pH 8.0, 30°C, recombinant mutant enzyme E314A |
730749 |
3.1.4.52 | 0.00077 |
- |
5'-phosphoguanylyl(3'->5')guanosine |
pH 8.0, 30°C, recombinant wild-type enzyme |
730749 |