EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
2.6.1.85 | 0.000098 |
- |
chorismate |
mutant K274R, cosubstrate NH4+, pH 8.5, 25°C |
661933 |
2.6.1.85 | 0.00083 |
- |
chorismate |
mutant K274R, cosubstrate L-glutamine, pH 8.5, 25°C |
661933 |
2.6.1.85 | 0.002 |
- |
chorismate |
mutant K274A, cosubstrate L-glutamine, pH 8.5, 25°C |
661933 |
2.6.1.85 | 0.007 |
- |
chorismate |
PabB, addition of Escherichia coli PabA enhances the reaction rate 20fold in the absence of glutamine, pH 8.0, 25°C |
724331 |
2.6.1.85 | 0.013 |
- |
L-glutamine |
pH 8.0, 25°C |
738864 |
2.6.1.85 | 0.017 |
- |
chorismate |
pH 8.0, 25°C |
738864 |
2.6.1.85 | 0.02 |
- |
2-amino-2-deoxychorismate |
PabB, second half of the reaction, pH 8.0, 25°C |
724331 |
2.6.1.85 | 0.026 |
- |
chorismate |
mutant K274A, cosubstrate NH4+, pH 8.5, 25°C |
661933 |
2.6.1.85 | 0.032 |
- |
chorismate |
wild-type, cosubstrate NH4+, pH 8.5, 25°C |
661933 |
2.6.1.85 | 0.12 |
- |
NH3 |
NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C |
724111 |