EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    2.4.2.19 | -999 |
- |
5-phospho-alpha-D-ribose 1-diphosphate |
Vmax = 0.93(+/-0.03) microM/min, wild-type, 0.3 mM quinolinic acid, UV-based assay measuring nicotinic acid mononucleotide formation at 266 nm, Vmax increases with increasing 5-phospho-alpha-D-ribose 1-diphosphate concentrations up to 5 mM |
693620 |
| 2.4.2.19 | -999 |
- |
more |
- |
638035 |
| 2.4.2.19 | -999 |
- |
more |
turnover rate is decreased by adding glycerol |
638030 |
| 2.4.2.19 | -999 |
- |
quinolinic acid |
Vmax = 0.71(+/-0.0) microM/min, mutant R161A, 0.1 mM 5-phospho-alpha-D-ribose 1-diphosphate, UV-based assay measuring nicotinic acid mononucleotide formation at 266 nm |
693620 |
| 2.4.2.19 | -999 |
- |
quinolinic acid |
Vmax = 1.19(+/-0.05) microM/min, wild-type, 0.1 mM 5-phospho-alpha-D-ribose 1-diphosphate, UV-based assay measuring nicotinic acid mononucleotide formation at 266 nm |
693620 |
| 2.4.2.19 | 0.00024 |
- |
quinolinic acid |
mutant E214A |
702358 |
| 2.4.2.19 | 0.00024 |
- |
quinolinic acid |
mutant R118A, at 25°C |
702359 |
| 2.4.2.19 | 0.00025 |
- |
quinolinic acid |
mutant E214D |
702358 |
| 2.4.2.19 | 0.0016 |
- |
quinolinic acid |
mutant K185A, at 25°C |
702359 |
| 2.4.2.19 | 0.03 |
- |
quinolinic acid |
mutant R152A, at 25°C |
702359 |
