EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
2.1.1.72 | -999 |
- |
more |
- |
485577, 659268, 659301, 659715 |
2.1.1.72 | -999 |
- |
more |
residues at position 20 and 26 are responsible for the different turnover-number values of the two MTases bor both canonical and noncanonical sites |
485577 |
2.1.1.72 | -999 |
- |
more |
turnover numbers for the canonical 14-mer duplex and various substituted duplexes |
485572 |
2.1.1.72 | -999 |
- |
more |
turnover-numbers of synthetic oligonucleotide substrates containing the native recognition site GATC or its modified variants |
485568 |
2.1.1.72 | -999 |
- |
more |
turnover-numbers of the canonical 12-mer and 20 mers and for various defective duplexes |
485573 |
2.1.1.72 | 0.000096 |
- |
DNA adenine |
- |
671878 |
2.1.1.72 | 0.000118 |
- |
S-adenosyl-L-methionine |
- |
671878 |
2.1.1.72 | 0.000167 |
- |
DNA adenine |
mutant S124D |
676217 |
2.1.1.72 | 0.00017 |
- |
DNA adenine |
using the non-preferred sequence 5'-GCATAAAAGATCGTCTAAATG-3', mutant enzyme N132A, in 20 mM potassium phosphate, pH 7.5, 200 mM NaCl, 0.2 mM EDTA, 0.2 mg/ml bovine serum albumin, 2 mM dithiothreitol, 10% (v/v) glycerol, at 22°C |
704502 |
2.1.1.72 | 0.0002 |
- |
double stranded DNA adenine |
with specific sequence AGGCTT/TCCGAA, at pH 7.0 and 37°C |
758441 |