EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.14.20.1 | 0.001 |
- |
penicillin G |
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258K |
440371 |
1.14.20.1 | 0.005 |
- |
ampicillin |
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258H |
440371 |
1.14.20.1 | 0.0057 |
- |
penicillin G |
wild-type enzyme, pH not specified in the publication, temperature not specified in the publication |
714589 |
1.14.20.1 | 0.007 |
- |
ampicillin |
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258H |
440371 |
1.14.20.1 | 0.008 |
- |
ampicillin |
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258A |
440371 |
1.14.20.1 | 0.009 |
- |
ampicillin |
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258L |
440371 |
1.14.20.1 | 0.009 |
- |
ampicillin |
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258K |
440371 |
1.14.20.1 | 0.01 |
- |
penicillin G |
pH 7.5, cosubstrate 2-oxoglutarate, recombinant mutant R258K |
440371 |
1.14.20.1 | 0.01 |
- |
penicillin G |
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258Q |
440371 |
1.14.20.1 | 0.011 |
- |
ampicillin |
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutants R258A and R258F |
440371 |