EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.13.11.20 | 0.003 |
- |
3-Mercaptopropanoate |
mutant G95C, PIPES buffer pH 6.5, 37°C |
758787 |
1.13.11.20 | 0.01 |
- |
3-Mercaptopropanoate |
pH 6.5, 30°C |
741704 |
1.13.11.20 | 0.01 |
- |
L-cysteine |
pH 6.5, 30°C, recombinant His-tagged enzyme |
741704 |
1.13.11.20 | 0.011 |
- |
3-Mercaptopropanoate |
wild-type, PIPES buffer pH 6.1, 37°C |
758787 |
1.13.11.20 | 0.012 |
- |
L-cysteine |
assay standart conditions are: the enzyme is incubated at 37°C in the presence of 62.5 mM Mes buffer (pH 6.1), 0.3 mM ferrous sulfate, 0.0125 mM bathocuproine disulfonate and 1.2 mM cysteine |
676962 |
1.13.11.20 | 0.013 |
- |
3-Mercaptopropanoate |
wild-type, phosphate buffer pH 8.0, 37°C |
758787 |
1.13.11.20 | 0.019 |
- |
3-Mercaptopropanoate |
wild-type, PIPES buffer pH 7.5, 37°C |
758787 |
1.13.11.20 | 0.021 |
- |
3-Mercaptopropanoate |
pH 7.5, 37°C |
759471 |
1.13.11.20 | 0.021 |
- |
3-Mercaptopropanoate |
wild-type, phosphate buffer pH 7.5, 37°C |
758787 |
1.13.11.20 | 0.022 |
- |
3-Mercaptopropanoate |
wild-type, PIPES buffer pH 6.5, 37°C |
758787 |