EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    5.3.2.6 | 1330 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
at pH 7.3 and 22°C |
747782 |
| 5.3.2.6 | 0.4 |
- |
2-hydroxy-2,4-hexadienedioate |
below, pH 7.3, 23°C, mutant R11A/R39A |
718830 |
| 5.3.2.6 | 2890 |
- |
2-oxo-4(E)-hexenedioate |
D-4OT enantiomer, pH 7.3, 30°C |
719636 |
| 5.3.2.6 | 107 |
- |
5-(methyl)-2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
| 5.3.2.6 | 4600 |
- |
2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
| 5.3.2.6 | 3.4 |
- |
5-(methyl)-2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
| 5.3.2.6 | 18.5 |
- |
2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
| 5.3.2.6 | 2940 |
- |
2-oxo-4(E)-hexenedioate |
L-4OT enantiomer, pH 7.3, 30°C |
719636 |
| 5.3.2.6 | 32 |
- |
2-hydroxymuconate |
mutant 4-OT I52E, pH 7.3, 23°C |
718844 |
| 5.3.2.6 | 61 |
- |
2-hydroxymuconate |
mutant 4-OT L8R, pH 7.3, 23°C |
718844 |
