EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   4.1.3.43 | -999 |
- |
more |
2 and 5fold increases in kcat observed when BphI reaction is conducted in the presence of NAD+ and NADH |
721615 |
| 4.1.3.43 | 0.4 |
- |
glycolaldehyde |
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C |
702377 |
| 4.1.3.43 | 0.86 |
- |
acetaldehyde |
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C |
702377 |
| 4.1.3.43 | 1.79 |
- |
propionaldehyde |
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C |
702377 |
| 4.1.3.43 | 3.9 |
- |
4-hydroxy-2-oxohexanoate |
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
| 4.1.3.43 | 4.1 |
- |
4-hydroxy-2-oxopentanoate |
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
| 4.1.3.43 | 2.1 |
- |
4-hydroxy-2-oxohexanoate |
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
| 4.1.3.43 | 2.3 |
- |
4-hydroxy-2-oxopentanoate |
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
| 4.1.3.43 | 1.9 |
- |
4-hydroxy-2-oxohexanoate |
G322A mutant, steady state, pH 8.0, 25°C |
721651 |
| 4.1.3.43 | 2.4 |
- |
4-hydroxy-2-oxopentanoate |
G322A mutant, steady state, pH 8.0, 25°C |
721651 |
