EC Number   |
Turnover Number Minimum [1/s]  |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   4.1.3.43 | -999 |
- |
more |
2 and 5fold increases in kcat observed when BphI reaction is conducted in the presence of NAD+ and NADH |
721615 |
| 4.1.3.43 | -999 |
- |
more |
value not detectable for R16A variant of BphI |
714244 |
| 4.1.3.43 | -999 |
- |
more |
with 4-hydroxy-2-oxopentanoate at 50°C turnover number is increased approximately 4fold |
721659 |
| 4.1.3.43 | 0.043 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
| 4.1.3.43 | 0.05 |
- |
oxaloacetate |
L87N variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
| 4.1.3.43 | 0.08 |
- |
4-hydroxy-2-oxopentanoate |
with Ca2+ chloride salt, pH 8.0, 25°C |
721659 |
| 4.1.3.43 | 0.08 |
- |
oxaloacetate |
L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
| 4.1.3.43 | 0.089 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
| 4.1.3.43 | 0.098 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
| 4.1.3.43 | 0.099 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
